Answered

Find the best answers to your questions at Westonci.ca, where experts and enthusiasts provide accurate, reliable information. Experience the ease of finding quick and accurate answers to your questions from professionals on our platform. Get quick and reliable solutions to your questions from a community of experienced experts on our platform.

you have cloned a gene for an enzyme that degrades lipids in a bacterium that normally lives in cold temps. you wish to use this gene in E. coli to produce industrial amounts of enzyme for use in laundry detergent.
a. how would you accomplish this?
b. you have managed to produce transgenetic E. coli expressing mRNA of your gene, but only a low level of protein is produced. why might this be so? how can you overcome this problem?

Sagot :

A) You would have to incorporate that gene into the bacteria's plasmid (genetic information that isn't in its main genome) so that it can use it and express its message. This is done using enzymes that cut that circular plasmid, insert the gene you want, and put the circular molecule back together. Once you have the bacteria with the plasmid in it, you replicate that bacteria, so all the resulting copies will have that gene and they'll express it.

B) If not enough protein is being produced it could be because you don't have enough bacteria, you'd need a bigger population. The medium the bacteria is in also should be optimal so that it can be as efficient as possible.
We hope you found this helpful. Feel free to come back anytime for more accurate answers and updated information. We hope our answers were useful. Return anytime for more information and answers to any other questions you have. We're here to help at Westonci.ca. Keep visiting for the best answers to your questions.