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Sagot :
Answer:
I would pipet 4 μl of each BSA work solution
Explanation:
To calculate the mass (in μg), we have to multiply the concentration C (in μg/ul) by the volume V (in μl):
C(μg/ul) x V( μl) = mass (μg)
We found the required volume for each solution, as follows:
5 μg/μl x V₁ = 20 μg ⇒ V₁ = 20 μg/(5 μg/μl ) = 4 μl
0.5 μg/μl x V₂ = 2 μg ⇒ V₂ = 2 μg/(0.5 μg/μl) = 4 μl
0.05 μg/μl x V₃ = 0.2 μg ⇒ V₃= 0.2 μg/(0.05 μg/μl) = 4 μl
Therefore, we need to pipet 4 μl of the solutions of concentrations 5μg/μl, 0.5 μg/μl, and 0.05 μg/μl to obtain the masses 20 μg, 2 μg, and 0.2 μg, respectively.
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