Answered

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A student makes a new gel using agarose, water, and buffer solution. The student loads their DNA samples in their wells and places the gel in the chamber for an appropriate length of time. When the student places the gel on the UV lightbox, no DNA bands show up at all. Even the marker lane is clear and has no bands! What mistake did the student make? Explain your answer.

Sagot :

luisvaschetto

Answer:

A dye needs to be added to the gel electrophoresis

Explanation:

Ethidium bromide (EtBr) is a reagent that adheres to DNA and fluoresces under ultraviolet (UV) light. Due to this feature, EtBr is widely used to dye DNA strands in molecular biology techniques such as agarose gel electrophoresis. The most common dyes for visualizing and staining nucleic acids during gel electrophoresis include EtBr, SYBR gold, Eva green and SYBR safe. If EtBr (or any other staining agent) was not added to the electrophoresis gel and/or running buffer, the gel will be dark under UV light (even the ladder lane will look empty) because DNA has not been stained.

adefioyelaoye

The mistake the student made was by not adding dye to the sample. Dyes

are commonly used during electrophoresis.

Agarose gel electrophoresis is the process which helps in the separation

and purification of DNA samples. This process involves the use of dyes

which acts as markers.

in this scenario, we were told no DNA bands show up and the marker lane is

clear means no dye was added.  The common dye used in agarose gel

electrophoresis is Ethidium bromide (EtBr) which should be added by the

student.

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