Get the answers you need at Westonci.ca, where our expert community is dedicated to providing you with accurate information. Discover in-depth solutions to your questions from a wide range of experts on our user-friendly Q&A platform. Experience the convenience of finding accurate answers to your questions from knowledgeable experts on our platform.
Sagot :
Answer:
a. At pH 9, the product, p-nitrophenol, will be ionized, the solution will appear yellow in color, and thus can be monitored at the wavelength of maximum absorption for the phenolate ion which is 400nm
Explanation:
In alkaline phosphatase assay, the hydrolysis of p nitrophenyl phosphate to p nitrophenol happens. When the ph is 9, the product which is p nitrophenol would undergo ionization. The solution is going to appear to be of yellow and it can be monitored at a wavelength for maximum absorption of phenolate ions at 400nm.
Option A is the answer to the question.
We hope our answers were helpful. Return anytime for more information and answers to any other questions you may have. Thank you for your visit. We're dedicated to helping you find the information you need, whenever you need it. Get the answers you need at Westonci.ca. Stay informed by returning for our latest expert advice.