Answered

Discover answers to your most pressing questions at Westonci.ca, the ultimate Q&A platform that connects you with expert solutions. Get quick and reliable answers to your questions from a dedicated community of professionals on our platform. Discover detailed answers to your questions from a wide network of experts on our comprehensive Q&A platform.

You created three different derived strains from E.coli K12. One strain includes a deletion in a gene critical for biosynthesis of diaminopimelic acid, one strain lacks dnaQ, and the last strain lacks both. Describe experiments to clearly differentiate between these three strains through phenotypic assays alone (not just by sequencing the genomes or any derivative of genotyping). Include all necessary controls to interpert the experiment.

Sagot :

batolisis

Answer:

For the strain that lacks DNAQ there will be slowed growth and there will more DNA breakage by Tunel assay

For the strain that lacks both The cells there will have a huge DNA breakage and less peptidoglycan.

Explanation:

One of the experiments that can be used to differentiate diaminopimelic acid is by observing the cell walls in which they are present because  are needed in the formation of cell walls. while to differentiate the three strains

For the strain that lacks DNAQ there will be slowed growth and there will more DNA breakage by Tunel assay

For the strain that lacks both The cells there will have a huge DNA breakage and less peptidoglycan.

Thanks for stopping by. We are committed to providing the best answers for all your questions. See you again soon. Thank you for your visit. We're dedicated to helping you find the information you need, whenever you need it. We're here to help at Westonci.ca. Keep visiting for the best answers to your questions.