Answered

Discover the answers to your questions at Westonci.ca, where experts share their knowledge and insights with you. Experience the convenience of getting accurate answers to your questions from a dedicated community of professionals. Connect with a community of professionals ready to help you find accurate solutions to your questions quickly and efficiently.

You created three different derived strains from E.coli K12. One strain includes a deletion in a gene critical for biosynthesis of diaminopimelic acid, one strain lacks dnaQ, and the last strain lacks both. Describe experiments to clearly differentiate between these three strains through phenotypic assays alone (not just by sequencing the genomes or any derivative of genotyping). Include all necessary controls to interpert the experiment.

Sagot :

batolisis

Answer:

For the strain that lacks DNAQ there will be slowed growth and there will more DNA breakage by Tunel assay

For the strain that lacks both The cells there will have a huge DNA breakage and less peptidoglycan.

Explanation:

One of the experiments that can be used to differentiate diaminopimelic acid is by observing the cell walls in which they are present because  are needed in the formation of cell walls. while to differentiate the three strains

For the strain that lacks DNAQ there will be slowed growth and there will more DNA breakage by Tunel assay

For the strain that lacks both The cells there will have a huge DNA breakage and less peptidoglycan.

We hope our answers were useful. Return anytime for more information and answers to any other questions you have. Thank you for choosing our platform. We're dedicated to providing the best answers for all your questions. Visit us again. Find reliable answers at Westonci.ca. Visit us again for the latest updates and expert advice.