In a separate line of experiments, you are studying a protein that you suspect shuttles between the nucleus and the cytoplasm. However, when you localize a GFP-tagged fusion protein, it appears to be only in the nucleus. To test whether it shuttles, you set up a heterokaryon assay. You take a cell expressing the GFP fusion protein and partially fuse that cell with another that does not express the GFP protein. The result is a heterokaryon that has a single cytoplasm but two separate nuclei. You treat the heterokaryon with the translation inhibitor cycloheximide for several hours and then examine the localization of the GFP protein. What do you predict will be observed if the GFP protein shuttles between the nucleus and the cytoplasm

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08-03-2021
Biology

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In a separate line of experiments, you are studying a protein that you suspect shuttles between the nucleus and the cytoplasm. However, when you localize a GFP-tagged fusion protein, it appears to be only in the nucleus. To test whether it shuttles, you set up a heterokaryon assay. You take a cell expressing the GFP fusion protein and partially fuse that cell with another that does not express the GFP protein. The result is a heterokaryon that has a single cytoplasm but two separate nuclei. You treat the heterokaryon with the translation inhibitor cycloheximide for several hours and then examine the localization of the GFP protein. What do you predict will be observed if the GFP protein shuttles between the nucleus and the cytoplasm

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codiepienagoya codiepienagoya · Answer 1 · 2021-03-11T00:45:14-05:00

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Explanation:

The heterokaryons were cells of diverse traditions or more nuclei. A heterokaryon cell with network cells (donor and receiver nuclei) sharing a common costly to treat is produced whenever a node containing GFP-tagged protein (donor cell) is merged to a cell non express the GFP-tagged proteins (recipient cell). Unless the intracellular scram nuclear exists, GFP must leave the GFP atoms, be shipped to the cytosol, and be exported to a nucleus with emission of GFP protein (recipient nucleus). It is obtaining nuclear has been beginning to release the GFP protein.

Unless the GFP protein doesn't display a nucleus costly to treat, this is not distributed across time in the metal target. By either sole dissemination through nuclear pores or receptor-mediated routes, protein yelling from nuclear to emotional exhaustion can occur. That GFP proteins are shown in a nucleus only suggests a path via a transmitter. For chloroplast, though, protein disperses via nuclear envelope. It is not the case. It should have nuclear foreign trade signals when ferrying between the nucleus and cytoplasm.

Cycloheximide is indeed a medicine that stops protein expression without elongating. For heterokaryon cells, it inhibits fresh protein synthesis. It makes visualization of a nanoparticle cell of only old pre-existing molecules (before cycloheximide diagnosis). That post GFP substance is a shuttling shielding substance that passes seen between the nucleus and the cytoplasm and the receiver nucleus. Whenever the levels of the shuttle were high, the GFP protein expression is shown both by the sender and receiver nucleus. The recipient nucleus will not be left without any schlepping protein. Thus, the donor nucleus can only be used.

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