Amanda inserted the gel tray with the well towards the red ( ) end of the chamber during gel electrophoresis and carefully pipetted her samples into the wells as indicated in the lab directions. After viewing her gel under UV light, she did not find bands in the regions that she was expecting. What did she most likely see in her gel results and why

Question

01-04-2021
Chemistry

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Amanda inserted the gel tray with the well towards the red ( ) end of the chamber during gel electrophoresis and carefully pipetted her samples into the wells as indicated in the lab directions. After viewing her gel under UV light, she did not find bands in the regions that she was expecting. What did she most likely see in her gel results and why

Sagot :

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batolisis batolisis · Answer 1 · 2021-04-05T07:22:54-04:00

Answer:

hello the options related to your question is missing attached below is the complete question with the options

answer:

Any visible bands were found in the small area above the wells instead in the expected large bottom area of the gel, because Amanda should have placed the wells into the black (-) end of the chamber for DNA to move towards the opposite red (+) end of the gel. ( 2 )

Explanation:

Why Amanda did not see any bands in the region that she was expecting is because during electrophoresis ( which allows the distinguishing of various lengths of DNA fragments on a gel ) the DNA been negative will migrate to the positively charged electrodes and The shorter brands will move faster through the gel, providing DNA fragments arrangements on the gel according to size.

Sagot :

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