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Blue-white screening is a technique for the identification of___. It relies on the ability of____to cleave lactose. Blue-white tests take advantage of a molecule called____which is similar to lactose in that it is cleaved by B-galactosidase. When cleaved, the____(same as previous) turns . If uncleaved,meaning a non- function B-gal gene, the X-gal remains____. Therefore, a____bacterial colony means the B-galactosidase gene is not-functional, and therefore there____a recombinant gene inserted into the vector.
You’ve inserted a gene for pesticide resistance into your vector. To see if it was successful, you decide to conduct a blue-white screening test. But, uh-oh you are out of X-gal! You do notice some green Slime-gal sitting on the shelf and recall that this molecule works similarly to X-gal, but instead of turning blue when cleaved, it turns green when cleaved. You carry out the test, and find that all of your colonies are green! Was your insertion of the pesticide gene successful?

Sagot :

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Answer:

Explanation:

Blue-white screening is a method for distinguishing proof of (recombinant bacteria). It depends on the capacity of ( B-galactosidase) to separate lactose. Blue-white tests exploit the molecule called  (x-gel)_ which is like lactose in that it is severed by B-galactosidase. When separated, the (5-bromo-4-chloro-indoxyl) (same as past) turns _(_blue). In the event that uncleaved, which implies a non-function B-gal gene, the X-gal remains (white)_. Subsequently, a __(white) bacterial province implies the B-galactosidase gene isn't practical, and in this way there ___lacz__ a recombinant gene embedded into the vector.

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