You are tasked with characterizing responses to Epidermal Growth Factor (EGF) treatment of HeLa Cells. You grow HeLa cells then replace the culture media with media containing EGF at various concentrations, then harvest samples for Western blot analysis using an anti-phospho-MAPK antibody as a readout of receptor tyrosine kinase activity. As expected, your control (EGF treated cells) show a strong phopho-MAPK signal on your Western blot. What do you hypothesize will happen if you pre-treat HeLa cells with siRNAs (RNAi) against Ras prior to adding EGF and performing Western blot analysis with anti-phospho-MAPK

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05-04-2021
Biology

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You are tasked with characterizing responses to Epidermal Growth Factor (EGF) treatment of HeLa Cells. You grow HeLa cells then replace the culture media with media containing EGF at various concentrations, then harvest samples for Western blot analysis using an anti-phospho-MAPK antibody as a readout of receptor tyrosine kinase activity. As expected, your control (EGF treated cells) show a strong phopho-MAPK signal on your Western blot. What do you hypothesize will happen if you pre-treat HeLa cells with siRNAs (RNAi) against Ras prior to adding EGF and performing Western blot analysis with anti-phospho-MAPK

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codiepienagoya codiepienagoya · Answer 1 · 2021-04-09T02:29:25-04:00

Answer:

The answer is "The EGF receptor will be activated but the signal will not be appropriate transduced"

Explanation:

It induces the replication of cell types, especially fibroblasts or epithelial cells. It enhances neuron variety's telling the difference, ripening, and survival. The proinflammatory cytokines of the EGF family are described by four disulfide bonds that confer adhesive properties. The EGF sensor is switched on and the signal is not transmitted correctly.

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