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Answer parts A, B, C, D
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a). a microRNA (miRNA) is a small non-coding RNA involved in transcriptional and post transcriptional regulation of gene expression. Given the mRNA molecule below, suggest an example of a complementary 10 nucleotide miRNA. circle or highlight the sequence on the mRNA template to which your miRNA will bind, give the complementary 10 nucleotide sequence and clearly label the orientation of the miRNA molecule.
mRNA region: 5’-GUAGUACUGAUGGUCCAGUACGUGCUAGGUCGCUAGUAGUUCGAUGCAGUAUUAU-3’
b). what is the consequence of this miRNA binding to the mRNA above?
c). what happens when the miRNA is not completely complementary to the mRNA it is attached to?
d). what is one theory explaining the evolution of the RNAi pathway? what important research function has RNAi served since its discovery?
