Answered

Discover a wealth of knowledge at Westonci.ca, where experts provide answers to your most pressing questions. Explore our Q&A platform to find reliable answers from a wide range of experts in different fields. Join our platform to connect with experts ready to provide precise answers to your questions in different areas.

Fire and Mello conducted an experiment where they added no gene X RNA (b), anti-sense gene X RNA (c) or double stranded gene X RNA (d) to C. elegans embryos. They then did a hybridization experiment examining gene X mRNA levels in the embryo. Square (a) is a no probe control. How does this act as a control

Sagot :

Histrionicus

Answer:

The correct answer is - It shows that an RNA probe is necessary to get a signal.

Explanation:

A probe is a specific single-stranded sequence of DNA or RNA that recognizes its complementary sequence in a particular sample genome for synthesis. The RNA probe is placed into close contact with the given sample under a specific environment that allows the RNA probe sequence to hybridize with its complementary sequence.

In this case, square a is the condition where no RNA probe is persent whereas all three other conditions have some sort of RNA probe, however, in C there is no Gene X probe but their probe present. To get a signal in hybridization RNA probe is required and that can be used as control as it can be used to compare if a signal is received or not.

Thanks for using our service. We aim to provide the most accurate answers for all your queries. Visit us again for more insights. Thanks for using our platform. We aim to provide accurate and up-to-date answers to all your queries. Come back soon. We're glad you visited Westonci.ca. Return anytime for updated answers from our knowledgeable team.