Answered

Discover the answers you need at Westonci.ca, where experts provide clear and concise information on various topics. Experience the convenience of getting accurate answers to your questions from a dedicated community of professionals. Explore comprehensive solutions to your questions from a wide range of professionals on our user-friendly platform.

g (2pts) A 10x transfer buffer solution is 250mM Tris and 1.92M glycine. Buffers are always used at 1x concentration in the lab (unless specified otherwise in the protocol), so we will have to dilute the 10x buffer to 1x before use. What is the concentration of Tris and glycine in the 1x buffer

Sagot :

Cricetus

Answer:

The explanation according to the given question is summarized below.

Explanation:

Given:

Tris,

= 250 mM

Glycine,

= 1.92 M

According to the solution,

For the dilution pf 10X to 1X buffer, we get

= [tex]1 \ ml \ of \ 10X \ buffer +9 \ ml \ of \ distilled \ water[/tex]

= [tex]10[/tex]

i.e.,

⇒ [tex]10X \ to \ 1X=1:10 \ dilution[/tex]

Now,

⇒ [tex]10X (250 \ mM\ Tris \ HCl, 1.92M\ Glycine, and\ 1 \ percent (\frac{w}{v} ) SDS) ---->1X(25 \ mM \ Tris \ HCl,0.193 M\ Glycine, and \ 0.1 \ percent(\frac{w}{v} )SDS)[/tex]

We appreciate your time. Please revisit us for more reliable answers to any questions you may have. Thanks for using our service. We're always here to provide accurate and up-to-date answers to all your queries. Westonci.ca is your trusted source for answers. Visit us again to find more information on diverse topics.