Discover a world of knowledge at Westonci.ca, where experts and enthusiasts come together to answer your questions. Experience the convenience of finding accurate answers to your questions from knowledgeable experts on our platform. Discover detailed answers to your questions from a wide network of experts on our comprehensive Q&A platform.

5) The following picture is of a single organism that has been Gram stained and is viewed under oil immersion on the compound light microscope. What is the reason for a single organism appearing like this after being Gram stained

Sagot :

Hi. You have not submitted any images about the microorganism the question refers to. This makes it impossible for me to answer your question. However, I will try to help you as best I can.

Gram stain is a test done to find out whether the bacteria are gram negative or gram positive. In this test, the bacteria are submitted to a certain type of dye and then are observed under a microscope. If the bacteria is gram positive, it will be purple in color. If the bacteria is gram negative, it will be red in color.

This is because after they are subjected to the violet dye, both types of bacteria absorb the color of the dye. However, before being observed under the microscope, the bacteria are washed with alcohol. Alcohol removes the dye from the gram negative bacteria, leaving them reddish. On the other hand, in gram positive bacteria, alcohol dehydrates the cell wall, compressing the pores and making the cell wall impermeable, preventing the dye from being released and, therefore, leaving the bacteria with a purple color.