sarahhudson114
Answered

Westonci.ca offers fast, accurate answers to your questions. Join our community and get the insights you need now. Discover detailed solutions to your questions from a wide network of experts on our comprehensive Q&A platform. Get immediate and reliable solutions to your questions from a community of experienced professionals on our platform.

Genetic engineering involves various stages.

1 human DNA is inserted into bacterial plasmid DNA
2 recombinant plasmid inserted into bacteria
3 restriction enzyme cuts bacterial plasmid DNA
4 restriction enzyme cuts human DNA

What is the correct sequence for genetic engineering?​

Sagot :

adrianaivy22

Answer:

4, 3, 1, 2

Explanation:

FIRST: cDNA is synthesized to be used as a gene of interest: processed (spliced) mRNA (no introns) is reverse transcribed to cDNA using reverse transcriptase, an enzyme from retroviruses

SECOND: Restriction enzyme used to cut GOI

THIRD: Same Restriction enzyme used to cut plasmid (sticky ends are created)

FOURTH: GOI connects/hybridizes with plasmid due to base-pairing

FIFTH: DNA ligase connects the recombinant DNA strands

Thank you for visiting our platform. We hope you found the answers you were looking for. Come back anytime you need more information. Thanks for using our service. We're always here to provide accurate and up-to-date answers to all your queries. Westonci.ca is committed to providing accurate answers. Come back soon for more trustworthy information.