Genetic engineering involves various stages.

1 human DNA is inserted into bacterial plasmid DNA
2 recombinant plasmid inserted into bacteria
3 restriction enzyme cuts bacterial plasmid DNA
4 restriction enzyme cuts human DNA

What is the correct sequence for genetic engineering?

Question

04-08-2021
Biology

Answered

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Genetic engineering involves various stages.

1 human DNA is inserted into bacterial plasmid DNA
2 recombinant plasmid inserted into bacteria
3 restriction enzyme cuts bacterial plasmid DNA
4 restriction enzyme cuts human DNA

What is the correct sequence for genetic engineering?

Sagot :

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adrianaivy22 adrianaivy22 · Answer 1 · 2021-08-04T01:05:46-04:00

Answer:

4, 3, 1, 2

Explanation:

FIRST: cDNA is synthesized to be used as a gene of interest: processed (spliced) mRNA (no introns) is reverse transcribed to cDNA using reverse transcriptase, an enzyme from retroviruses

SECOND: Restriction enzyme used to cut GOI

THIRD: Same Restriction enzyme used to cut plasmid (sticky ends are created)

FOURTH: GOI connects/hybridizes with plasmid due to base-pairing

FIFTH: DNA ligase connects the recombinant DNA strands

Genetic engineering involves various stages. 1 human DNA is inserted into bacterial plasmid DNA 2 recombinant plasmid inserted into bacteria 3 restriction enzyme cuts bacterial plasmid DNA 4 restriction enzyme cuts human DNAWhat is the correct sequence for genetic engineering?​

Sagot :

Other Questions

Genetic engineering involves various stages.

1 human DNA is inserted into bacterial plasmid DNA
2 recombinant plasmid inserted into bacteria
3 restriction enzyme cuts bacterial plasmid DNA
4 restriction enzyme cuts human DNA

What is the correct sequence for genetic engineering?