Answer:
A virus or plasmid used in this way is known as a cloning vector, and the DNA propagated by insertion into it is said to have been cloned. To isolate a specific gene, one often begins by constructing a DNA library—a comprehensive collection of cloned DNA fragments from a cell, tissue, or organism.
Restriction enzymes are used to isolate and cut the target gene during genetic engineering. Thus, the correct option is D.
Genetic engineering may be defined as a methodology that utilizes laboratory-based technologies to change the DNA makeup of an organism in a sequential manner.
Ligase is an enzyme that is utilized to join the fragments of a restriction endonuclease that have annealed in recombinant DNA cloning. Polymerases are also the class of enzymes that are used in DNA manipulation in vitro.
A Recognition site is a specific site that is usually very short in length and recognizes the action of enzymes and primers.
Therefore, the correct option for this question is D.
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