If Cas9 edits DNA, why is a molecule of RNA needed in a CRISPR complex?

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06-09-2021
Biology

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If Cas9 edits DNA, why is a molecule of RNA needed in a CRISPR complex?

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yashonamaharjan5 yashonamaharjan5 · Answer 1 · 2021-09-06T01:56:06-04:00

yashonamaharjan5 yashonamaharjan5

06-09-2021

Explanation:

The use of CRISPR/Cas9 avoids the need for protein engineering to develop a site-specific nuclease against a specific DNA target sequence, requiring only the synthesis of a new piece of RNA. This dramatically simplifies and greatly reduces the time needed for gene editing design and implementation.

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If Cas9 edits DNA, why is a molecule of RNA needed in a CRISPR complex?

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