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A chromatogram has three bands with Rf values of 0.25, 0.47 and 0.82 which value corresponds to the material with the highest molecular weight

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The compound that has the highest molecular weight based on the chromatogram is the compound with Rf 0.25.

A chromatogram is an output that shows the extent of separation of the components of a mixture. If the Rf is high, then it means that the substance traveled a large distance through the plate and vice versa.

Similarly, the molecular mass of the substances correlate with the Rf value observed for each component. The components with high molecular mass always show a low Rf value.

This implies that the lowest Rf value corresponds to the largest relative molecular mass. Thus, the compound that has the highest molecular weight based on the chromatogram is the compound with Rf 0.25.

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