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The pedigree chart below shows the individuals in a family who exhibit a certain trait. Based on the information in the chart, which of the following provides the best evidence that this is a recessive trait?
Key:
Circle—Female
Square—Male
Green—Exhibits trait"
Couple 3–4 in the 2nd generation produced children who do not show the trait.
Couple 1–2 in the 2nd generation produced children who do show the trait.
Couple 3–4 in the 2nd generation produced children who are identical twins.
Couple 1–2 in the 1st generation produced children who do show the trait.
