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Determining the order of the nucleotides within a gene is known as DNA sequencing. The earliest DNA sequencing methods were time consuming, but a major breakthrough came in 1975 with the development of the process called Sanger sequencing. Sanger sequencing is named after English biochemist Frederick Sanger, and it is sometimes also referred to as chain-termination sequencing or dideoxy sequencing. Some 25 years after its creation, the Sanger method was used to sequence the human genome, and, with the addition of many technological improvements and modifications, it remains an important method in laboratories across the world today.
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