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A column is run with hexane first, then a hexane/ dichloromethane mixture, then 100% dichloromethane. What will happen is you mix up the hexane and dichloromethane i.e. ran the dichloromethane first?

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ajeigbeibraheem

If hexane and dichloromethane are being mixed up together i.e if dichloromethane is added first, the impurities will mix together and it will be difficult and unable to separate.

Hexane and dichloromethane are two different solvents with different properties used in column chromatography. While hexane is polar in nature dichloromethane occurs to be a non-polar solvent.

The best way to run this process in the column is to first add hexane, followed by gradual addition of dichloromethane. This will help to separate and distinguish each compound in the mixture.

However, if dichloromethane is added first, the compound will get eluted and there wlll be no separation. The only solution will be to evaporate the solvent and run the column once again with the steps explained above.

Learn more about column chromatography here:

https://brainly.com/question/13542844?referrer=searchResults

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