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when preparing pure cultures, dilution is necessary for

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Dilution is necessary during pure culture preparation to reduce the bacterial load and allow readable colonies to grow.

Dilution of bacteria culture is carried out in comparison to McCfarlan standard so as to have a decent number of bacteria that will not be too numerous or too little to observe the growth of distinct colonies during culture preparation.

Without dilution, colonies may form but may be too numerous to count. Too much dilution may also lead to no growth in the culture plates.

More on pure culture preparation can be found here: https://brainly.com/question/9886600?referrer=searchResults

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