Given what we know, we can confirm that the colonies in quadrant 4 are larger in diameter than the colonies in quadrant 2, which may be caused by improper flaming of the loop.
In microbiology, a loop is also referred to as an inoculation wand. It is the tool used to create the smears that we will be studying. Flaming this loop in between smears is extremely important to prevent bacteria from being carried over from one smear to the next. This may have been the case in the experiment, causing colony four to be much larger than the rest.
Therefore, we can confirm that due to a lack of flaming to sterilize the loop in between smears, contamination may have occurred and therefore colony four is larger than the rest.
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