In the serial dilution for the preparation of the dye, the total dilution factor is 1/20
What is dilution of solution?
Dilution is the process of preparing solutions of lesser concentration from a more concentrated solution.
In test tube 1:
Total volume = 60 mL
Dilution factor = 1:4
1 parts stock solution is required for every four parts diluent
Total parts = 5
volume of diluent = 4/5 × 60 mL = 48 mL
48 mL of diluent is added to 12 mL of stock solution to prepare the solution in test tube 1.
In test tube 2:
Total volume = 15 mL
Dilution factor = 1:5
1 parts stock solution is required for every five parts diluent
Total parts = 6
volume of diluent = 5/6 × 15 mL = 12.5 mL
12.5 mL of diluent is added to 2.5 mL of stock solution from tube 1 to prepare the solution in test tube 2.
Total dilution factor = 1/4 × 1/5 = 1/20
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