jeslinrose
Answered

Discover the answers to your questions at Westonci.ca, where experts share their knowledge and insights with you. Connect with a community of experts ready to help you find accurate solutions to your questions quickly and efficiently. Our platform provides a seamless experience for finding reliable answers from a network of experienced professionals.

The enzyme provided is a stock solution which should be diluted 50-fold with 0.001M HCl immediately before use.

Enzyme :1mg/ml chymotrypsin A in 0.001M HCl



How do I work out this calculation, if I want the 5ml of the diluted enzyme?

Sagot :

mickymike92

In order to prepare a 50-fold diluted enzyme, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl.

What is dilution?

Dilution is a process whereby a solution of lower concentration is prepared from one of a higher concentration.

Dilution is done by using the dilution formula below:

  • C1V1 = C2V2

C1; Initial concentration of enzyme = 1 mg/mL

C2; Final concentration of enzyme = 1/50 mg/ml = 0.02 mg/ml

V1; Initial volume of enzyme = y

V2; Final volume = 5.0 ml

V1 = C2V2/C1

V1 =  0.02 * 5/1 = 0.1 mL

Therefore, in order to prepare a 50-fold diluted enzyme, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl.

Learn more about dilution at: https://brainly.com/question/24881505

#SPJ1

Thank you for visiting our platform. We hope you found the answers you were looking for. Come back anytime you need more information. Thanks for using our service. We're always here to provide accurate and up-to-date answers to all your queries. Keep exploring Westonci.ca for more insightful answers to your questions. We're here to help.