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A researcher has sequenced a gene that is present in two alleles. She has a mixture of both alleles and clones them into e. Coli using plasmids that contain the lacz gene. She is interested in one particular allele that is indicated in a disease. Using media containing x-gal, how can she select those colonies that have her allele of interest?.

Sagot :

She will need to sequence the plasmids of the white colonies before she can determine which allele it carries.

What are plasmids?

  1. A plasmid is a single-celled, circular, double-stranded DNA molecule that differs from chromosomal DNA.
  2. Plasmids are naturally prevalent in bacterial and eukaryotic cells. Plasmid genes usually provide bacteria with genetic benefits such as antibiotic resistance.
  3. RNA plasmids are uncommon, and the vast majority of them are unknown.
  4. Examples include plants, fungi, and even animals. Some strains of the yeast Saccharomyces cerevisiae contain linear RNA plasmids.
  5. Similar RNA plasmids have been identified in the mitochondria of different varieties of maize plants.

Read more about plasmids here:

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