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The original sample must be refilled and the most probable number (MPN) approach must be used if each dilution of the sample yields too few plates to count.
What is the most probable number(MPN) method?
The most probable number (MPN), a statistical technique based on the idea of extinction dilution, is used to calculate the number of bacteria that are likely to be present in a sample. It is frequently used to estimate the number of bacteria in food and water. However, the technique can only count living creatures. According to Food and Drug Agency (FDA) guidelines, which state that the maximum allowable limit in seafood is 10,000 cells per gram, it is imperative to count V. parahaemolyticus from seafood.
Why is the MPN technique crucial?
When samples include particulate matter that obstructs plate count enumeration techniques, MPN(most probable number) tests are particularly helpful. In order to determine if the water is safe to drink in terms of the number of bacteria present, MPN is most frequently used to assess the quality of water.
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