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Sagot :
TAND-1 and TAND-2 of TAF-1 blocks the DNA binding cleft of TPB and the n-terminal stirrup.
Explain function of TAF(I).
- Part of a protein known as transcription factor IID can be made using instructions from the TAF1 gene (TFIID).
- The body's cells and tissues all have this protein, which connects to and binds to DNA there.
- The majority of genes' activity is controlled by transcription factor IID.
- A preinitiation complex made up of a number of initiation components must be assembled before RNA polymerase II can begin transcription of a gene from its core promoter region.
- It is believed that the TFIID transcription factor complex, which is made up of the TATA-binding protein (TBP) and TBP-associated factors (TAF(II)s), binds to the various promoters in a sequence-specific manner to initiate the assembly of this complex.
- Here, we identify and characterize a novel multiprotein complex made up of a number of TAF(II)s and other proteins but lacking either TBP or a TBP-like factor.
- In in vitro transcription experiments, this complex can take the place of TFIID on both TATA-containing and TATA-lacking promoters.
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