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Explain why a glucose test strip may be used to determine the enzymatic activity of lactase

Sagot :

The glucose test strip may be used in the determination of the enzymatic activity of lactase

Since measuring the amount of generated glucose shows that the enzymes are actually converting the disaccharide into galactose and glucose, the glucose test strip can be used to determine the enzymatic activity of lactase.

As a byproduct of lactase hydrolysis, glucose serves as a direct indicator of the amount of lactase that has been reacted. Utilizing test strips that gauge the amount of glucose present in the reaction mixture, you can keep an eye on the enzyme activity.

An artificial substrate for lactase is hydrolyzed to extract lactase from dietary supplement tablets, which is then measured using a colorimetric assay (ortho-nitrophenol-beta-D-galactopyranoside, ONPG)

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