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Sagot :
The result consistent with the intestine actively transporting glucose from mucosal to serosal side, with a critical need for an apical sodium/potassium ATPase is that the glucose concentrations are higher on the serosal than mucosal at the end of the experiment unless ouabain is applied to the apical side.
The small intestine epithelium is electrogenic in its glucose absorption. The Na+/glucose cotransporter (SGLT1), although glucose transporter type 2 (GLUT2) may also be involved, is the main pathway for the transportation of dietary glucose from the intestinal lumen into enterocytes.
The steroid hormone ouabain is an alpha-L-rhamnosyl cardenoloide that has undergone multiple hydroxylation. It attaches to and prevents the sodium/potassium-ATPase in the plasma membrane. The binding reduces the enzyme's activity, which modifies the cell's transmembrane electrochemical potential.
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