Meiosis's pachytene substage of prophase I is when crossing over occurs, but the diplotene substage of prophase I is when it becomes evident. Tetrads will be formed when the homologous chromosomes align with one another.
DNA sequences may be swapped between the homologous chromosomes during this lining up. It facilitates character mixing, resulting in the production of both parental and recombinant forms. Crossing over is the term for the form of genetic recombination that enables the meiotic daughter cells to differ genetically from one another.
Only chromosomes that are homologous can cross across. After meiosis I, cells turn haploid and are no longer capable of crossing across.
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