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While the heterotrophic pate count technique will give us a good idea of the number of microbes found in the food sample the actual number of those present in this sample could be much higher. why?

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Only the culturable organisms in the sample, which can represent as little as 1% of the total bacteria present, are indicated by the heterotrophic plate count.

What is heterotrophic pate count technique?

A variety of factors can influence how bacteria grow on a given media. These variables include the make-up of the incubation medium, incubation temperature, incubation period, etc. Fastidious bacteria, for example, are unable to grow on the Nutrient Agar medium, and some germs can take a very long period to form colonies that are visible.

The actual number of microorganisms in the sample may be significantly higher than that estimated by a heterotrophic plate count, for the two primary reasons mentioned above.

Thus, Only the culturable organisms in the sample, which can represent as little as 1% of the total bacteria

For more details about  heterotrophic pate count technique, click here:

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