There will be little to no DNA
Taq DNA polymerase is the most common enzyme used for PCR amplification. This enzyme is extremely heat resistant with a half-life of 40 minutes at 95°C. At its optimal temperature (72°C), nucleotides are incorporated at a rate of 2–4 kilobases per minute.
What is PCR reaction ?
In PCR, a segment of the genome to be amplified is chosen using short synthetic DNA fragments called primers. Multiple rounds of DNA synthesis are then used to amplify that segment
- A specific target DNA sequence can be exponentially amplified using the Polymerase Chain Reaction (PCR), which enables the isolation, sequencing, or cloning of a single sequence among many.
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