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Why did we use a primary antibody that recognizes the xpress epitope tag and not the his6 tag to detect rgfp?

Sagot :

we use primary antibodies that recognize the Xpress epitope tag and not the his6 tag to detect rGFP because using antibodies that can detect his-tag will get bind to it.

Your immune system creates defensive proteins called antibodies. They bind to antigens (foreign substances), which include poisons, viruses, bacteria, and fungus, and eliminate them from your body. Your immune system creates defensive proteins called antibodies. They bind to antigens (foreign substances), which include poisons, viruses, bacteria, and fungus, and eliminate them from your body.

A protein called an antibody is created by your immune system to attack and neutralize antigens. The B cell produces antibodies (specialized white blood cells). A B cell multiplies and clones when it comes into touch with an antigen. These cloned B cells, also known as plasma cells, unleash countless quantities of antibodies into your lymphatic and blood systems.

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