The indicator stain in this microscope slide will be localized in organelles.
What are indicators?
- Substances called indicators are those whose solutions change color as the pH changes. We refer to these as acid-base indicators. The conjugate base or acid versions of these typically weak acids or bases have various hues because of variations in their absorption spectra.
- Organic weak acids or bases with complex structures are indicators. To assess the pH (acidity or basicity) of a solution visually, a little amount of a halochromic chemical substance is added to the solution.
- Therefore, under the Arrhenius model, a pH indicator is a chemical detector for hydronium ions (H3O+) or hydrogen ions (H+). According to the pH, the indicator typically causes the color of the solution to change. Other physical attributes can also alter, as seen by indicators.
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