Methylene blue can stain the cellular components like nucleus, DNA or RNA in cytoplasm.
Use of methylene blue:
- Methylene Blue is a cationic stain. It is a positively charged blue dye that binds to negatively charged cell components like the nucleus, DNA, or RNA found in the cytoplasm.
- It is a widely used stain for several purposes, including identifying germs and determining the cellular makeup of both plant and animal cells.
- The cell wall and membrane are examples of the exterior cell structures that can be stained by gram staining.
- In a collection of bacterial colonies, this stain is used to distinguish between gram positive and gram negative cells.
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