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Sagot :
Numerous animals navigate and interact with their surroundings by moving their limbs in unison. We mapped synaptic connections throughout a neural network that regulates limb motions using serial-section electron microscopy (EM) in order to understand the circuit principles underlying locomotor behavior. We offer a dataset of the ventral nerve cord (VNC) of an adult female Drosophila melanogaster with synapse resolution. We created GridTape, a method that combines automated serial-section collection with automated high-throughput transmission EM, to produce this dataset. We rebuilt 507 motor neurons using this dataset, including all of the ones in charge of the legs and wings. We demonstrate a new feedback system for quick limb control in which a certain class of leg sensory neurons directly synapses onto the largest-caliber motor neuron axons on both sides of the body. To enable the matching of cells between EM and light microscopy data, we make the dataset and reconstructions available to the public. To help the scientific community acquire large-scale EM data more easily and affordably, we also offer GridTape instrumentation designs and software.
To learn more about electron microscopy here:-
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