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Sagot :
The 0.5 % Azocasein is the substance used in enzyme assay. So the correct option is A.
What is an enzyme substrate?
A molecule with which an enzyme interacts is known as a substrate. A substrate is put into the active site of the enzyme, which is where weak bonds between two molecules might form. The substrate reacts under the pressure of the enzyme, forming an enzyme-substrate complex that is the product of the intended reaction.
The bonds that form between the substrate and the enzyme are what induce the conformational shift, or shape change, in the enzyme. The resulting shapeshift, which either pulls molecules together or splits them apart, is what exerts pressure on the substrate.
A substrate is a material that an enzyme uses in an enzymatic process. By reducing the activation energy required for the process, enzymes operate as biological catalysts to speed up chemical reactions.
In a chemical reaction, an enzyme is a protein that catalyzes the transformation of a substrate reactant into a product. In general, individual enzymes have a variety of substrates and may be tailored to a variety of reaction intermediates that are a part of a larger process.
Therefore the correct option is A.
Read more about substrates, here
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