What is the theoretical diffraction limit of resolution of a light microscope used to look at a sample through oil?

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06-09-2022
Biology

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What is the theoretical diffraction limit of resolution of a light microscope used to look at a sample through oil?

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vipinkumar69547 vipinkumar69547 · Answer 1 · 2022-09-09T02:23:36-04:00

The theoretical resolution limit of a light microscope being used examine a sample while it is in oil is 261 nm.

A light microscope's resolution is limited to between 0.4 and 0.7 micrometers, or half the wavelength of visible light. The objects are at most 0.2 m away when we can see green light (0.5 m).

Using a green light of 514 nm, an oil immersion objective with such a NA of 1.45, and a condenser with a NA of 0.95, the (theoretical) limit of resolution is 261 nm. As was already said, more detail can be resolved when imaging a specimen with a shorter wavelength of light in a microscope.

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