In the Gram staining procedure, acetone/alcohol is used for the purpose of color differentiation. In the presence of acetone/alcohol peptidoglycan molecules get shrunk in the cell wall for holding the crystal violet iodine tightly.
There might be no differentiation in Gram-advantageous or Gram-positive cells if they're decolorized with water instead of the acetone/alcohol answer. Peptidoglycan molecules do not shrink inside the aqueous surroundings.
Decolorizing will bring about an misguided end end result wherein gram-incredible cells might also stain red to purple indicating a gram-terrible cease result, and below-decolorizing will bring about a defective end result in which gram-terrible cells may additionally additionally appear blue to pink indicating a gram-splendid end result.
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