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sample source tube n dilution bradford reagent (ml) a595 dilution factor protein wheat germ inverse 10n wg1 100 2.5 2.50 1 wg2 1:10 10-1 2.5 1.74 10 wg3 1:100 10-2 2.5 0.46 100 wg4 1:1000 10-3 2.5 0.02 1000