The evidence Alfred Sturtevant use to support his conclusion that drosophila have four pairs of chromosomes was drosophila genes cluster into four distinct groups of linked genes.
Alfred Sturtevant worked on the frequency of recombination between gene pairs on the same locus of chromosomes as a sum of distance that exists between genes and mapped their positions on the chromosomes.
He is known for developed a technique for mapping the location of specific genes of the chromosomes in Drosophila. He also concluded that if a chromosome length is same in flies, and if genes had specific physical locations on it, the distance between any two genes should be a fixed number.
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