Question 1The pH of a solution describes its acidity or alkalinity: Describe how pH and H3O+ concentration are related and explain why diluting an acid raises the pH, but diluting a base lowers the pH.Question 2Phosphate Buffered Saline (PBS) is a commonly used buffer for experiments in biology because its pH and ion concentrations are similar to those in mammalian organisms. It works in a similar fashion to the blood plasma buffer mentioned in the textbook, but using dihydrogen phosphate ions and hydrogen phosphate ions for buffering through the following chemical reaction:H2PO4- (aq) ⇆ H+(aq) + HPO42--(aq)The equilibrium arrows depict that the phosphate ion (H2PO4- ) is dissociating further into two component ions in solution, but at the same time H+ and HPO42- ions are combining simultaneously to form phosphate in solution. So, at any given point in time, and under the appropriate conditions, there is an equal quantity of dissolved ions and combined ions in solution. There is therefore always a hydrogen ion donor and an acceptor in solution.Based on the equation above, which ion plays the role of hydrogen-ion donor (acid) and which ion plays the role of hydrogen-ion acceptor (base) in PBS?Question 3

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04-11-2022
Biology

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Question 1The pH of a solution describes its acidity or alkalinity: Describe how pH and H3O+ concentration are related and explain why diluting an acid raises the pH, but diluting a base lowers the pH.Question 2Phosphate Buffered Saline (PBS) is a commonly used buffer for experiments in biology because its pH and ion concentrations are similar to those in mammalian organisms. It works in a similar fashion to the blood plasma buffer mentioned in the textbook, but using dihydrogen phosphate ions and hydrogen phosphate ions for buffering through the following chemical reaction:H2PO4- (aq) ⇆ H+(aq) + HPO42--(aq)The equilibrium arrows depict that the phosphate ion (H2PO4- ) is dissociating further into two component ions in solution, but at the same time H+ and HPO42- ions are combining simultaneously to form phosphate in solution. So, at any given point in time, and under the appropriate conditions, there is an equal quantity of dissolved ions and combined ions in solution. There is therefore always a hydrogen ion donor and an acceptor in solution.Based on the equation above, which ion plays the role of hydrogen-ion donor (acid) and which ion plays the role of hydrogen-ion acceptor (base) in PBS?Question 3

Sagot :

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MaxmilianP153004 MaxmilianP153004 · Answer 1 · 2022-11-04T03:24:53-04:00

Question 2

Answer

H2PO4- is a hydrogen-ion donor (acid) and HPO42- is a hydrogen-ion acceptor (base).

Explanation

Given the equation:

[tex]H_2PO_{4(aq)}^-\text{ }\leftrightarrows\text{ H}_{(aq)}^+\text{ + HPO}_{4(aq)}^{2-}[/tex]

Required: Which ion plays the role of hydrogen-ion donor (acid) and which ion plays the role of hydrogen-ion acceptor (base) in PBS?

Solution:

H2PO4- is a hydrogen-ion donor (acid) and HPO4^2- is a hydrogen-ion acceptor (base).

In the equation above, the H2PO4- serves as a hydrogen donor, what happens is that Na2HPO4 dissociates into Na+ and HPO4-, this then results in a molecule being a proton acceptor. Where as in PBS solution, KH2PO4 dissociates into K+ and H2PO4-, this is a proton donor.

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