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You have carried out a genetic screen and isolated two pure-breeding lines of zebrafish with abnormal tail fin shapes. Both of the abnormal phenotypes are inherited in a recessive fashion. What is the easiest way to tell whether these two lines have mutations in the same gene or in two different genes?.

Sagot :

To carry out complementation take a look at, two homozygous individuals with similar mutant phenotypes are crossed (figure 4.6. 10). If the F1 progeny all have the identical mutant phenotype (Case 1 - parent 4.6. 10A), then we infer that the identical gene is mutated in each discern.

Mutation rate is calculated from the equation μ = m/N, in which N is the common range of cells per culture (approximately equal to the number of cell divisions in keeping with lifestyle for the reason that preliminary inoculum is a lot smaller than N).

A mutation is a trade in a genetic series. Mutations consist of adjustments as small as the substitution of an unmarried DNA building block, or nucleotide base, with some other nucleotide base. meanwhile, larger mutations can have an effect on many genes on a chromosome.

The handiest check to distinguish between the two possibilities is the complementation check. The take look is straightforward to perform ---  mutants are crossed, and the F1 is analyzed. If the F1 expresses the wild-kind phenotype.

Learn more about mutants  here:

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