once the modifications are made to the insulin dna, it is then prepared to be combined with the vector. but first, the dna must be cut in a way to allow for insertion. what enzyme cuts at specific sites in dna, leaving sticky ends ready for ligation? a) dna helicase b) dna polymerase iii c) dna exonuclease d) restriction endonuclease

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01-12-2022
Biology

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once the modifications are made to the insulin dna, it is then prepared to be combined with the vector. but first, the dna must be cut in a way to allow for insertion. what enzyme cuts at specific sites in dna, leaving sticky ends ready for ligation? a) dna helicase b) dna polymerase iii c) dna exonuclease d) restriction endonuclease

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The enzyme that cut DNA at a specific site to produce sticky ends is referred to as restriction endonuclease.

Restriction endonuclease is a type of enzyme that helps cleave the DNA into fragments at or near specific recognition sites within molecules called restriction sites. Since this enzyme cut the fragments within the molecule it is referred to as an endonuclease type of enzyme.

When the restriction enzyme first cuts on one end of the sequence, between two bases on the same strand, and then cuts on the other end of the complementary strand, a "sticky" end is created.

Two ends of DNA will result from this, and some nucleotides will have no complementary bases. This type of sticky ends thus helps to combine the target DNA with the vector during recombinant DNA technology.

Therefore, option d) restriction endonuclease is the correct option.

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