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Sagot :
Option (b) The donar will always possesses defective mismatch kind of repair mechanism, which allowing mutations to gradually try to accumulate.
Mutational inactivation of each copies of a DNA mismatch restore gene effects in a profound restore illness and revolutionary accumulation of mutations all through the genome. The DNA mismatch restore device is a bidirectional excision-resynthesis device this is initiated at a described strand scission this is 3′- or 5′- of a mismatch and this excision will tract extends to any nonspecific factor simply beyond the mismatch.
(MIS-healthy reh-PAYR deh-FIH-shun-see) Describes cells which have mutations (changes) in sure genes which can be worried in correcting errors made while DNA is copied in a cell. Mismatch restore (MMR) poor cells normally have many DNA mutations, which may also cause cancer.
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Correct Question:
Over time with several attempts of passaging, the Donor strain used in this laboratory exercise fails to successfully induce mutagensis in the Recicpient strain and eventually becomes difficult to culture. Based on your understanding of how the PRL27 plasmid behaves in the Donor strain, why do these things eventually occur?
a). The transpson is active in the donor, and mutations gradually accumulate due to continued activity.
b). The donor possesses defective mismatch repair mechanisms, allowing mutations to gradually accumulate.
c). The plasmid eventually integrates into the donor chromosome.
d). Pl protein slowly accumulates through time, resulting in overproduction of the plasmid.
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