2)where do restriction endonucleases come from? what is their natural purpose? 3)explain how a restriction endonuclease cuts dna. be sure to identify which type of bond in the dna molecule is broken. 4) what is meant by the term sticky ends? why are sticky ends useful in the research lab?

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01-12-2022
Biology

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2)where do restriction endonucleases come from? what is their natural purpose? 3)explain how a restriction endonuclease cuts dna. be sure to identify which type of bond in the dna molecule is broken. 4) what is meant by the term sticky ends? why are sticky ends useful in the research lab?

Sagot :

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contexto1220 contexto1220 · Answer 1 · 2022-12-06T03:44:31-05:00

Restriction endonucleases come from the Bacteria use limit enzymes to kill viruses – the enzymes assault the viral DNA and smash it into vain fragments.

Sticky ends are primarily used for cloning generation wherein a DNA ligase is used to enroll in DNA fragments into one. Sticky ends facilitate ligation with the aid of using DNA ligase with the aid of using forming hydrogen bonds among complementary bases of the opposite strand. Thus, the sticky ends are higher than the blunt ends.

When a limit endonuclease acknowledges a sequence, it snips thru the DNA molecule with the aid of using catalyzing the hydrolysis (splitting of a chemical bond with the aid of using addition of a water molecule) of the bond among adjoining nucleotides. Phosphodiester bond, DNA may be reduce with the aid of using limit endonucleases (RE).

Endonucleases are enzymes which could hydrolyze the nucleic acid polymer with the aid of using breaking the phosphodiester bond among the phosphate and the pentose at the nucleic acid backbone Restriction enzymes are located in bacteria.

2)where do restriction endonucleases come from? what is their natural purpose? 3)explain how a restriction endonuclease cuts dna. be sure to identify which type of bond in the dna molecule is broken. 4) what is meant by the term sticky ends? why are sticky ends useful in the research lab?

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