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Sagot :
The Xist transgene (Tg) integrated into the autosome can induce transcriptional silencing of neighboring genes. However, the effects and mechanisms of Xist RNA on autosomal sequence silencing remain elusive.
Xist or Tsix heterozygous mutations cause non-random X chromosome inactivation in female cells. When the expression of Xist increases from the X chromosome in cells before X chromosome inactivation, the X chromosome always becomes XI, and wild-type X always becomes XA. Xist encodes an RNA molecule that plays an important role in selecting the X chromosome to remain active, and the early spread and establishment of silencing on the inactive X chromosome allows the XIST RNA to enter interphase non-active cells. We smear the active X chromosome as evidence for the involvement of new RNAs. Nuclear/chromosomal structure. Inactivation of the X inactivation. chromosome is mediated by stabilization of the Xist RNA. Stabilization of Xist RNA mediates initiation of X inactivation.
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