which of the following techniques would not be suitable for determining the presence of a specific mrna in a tissue sample? a) rt-pcr b) rna interference c) rna-seq d) microarrays

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01-12-2022
Biology

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which of the following techniques would not be suitable for determining the presence of a specific mrna in a tissue sample? a) rt-pcr b) rna interference c) rna-seq d) microarrays

Sagot :

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tutorconsortium272 tutorconsortium272 · Answer 1 · 2022-12-06T01:56:33-05:00

Techniques that would not be suitable for determining the presence of a specific mRNA in a tissue sample are : RNA interference, RNA-seq, or microarray.

What techniques are suitable for determining the presence of a specific mRNA in a tissue sample?

The most sensitive technique for detecting and quantifying mRNA is RT- PCR. Using RT-PCR, even small sample sizes can be used in the quantification of mRNA and the technique can do this using just a single cell.

A number of widely used procedures exist for detecting and determining the abundance of a particular mRNA in RNA sample. But four popular methods are: Northern blot analysis, nuclease protection assays (NPA), in situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR).

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Sagot :

What techniques are suitable for determining the presence of a specific mRNA in a tissue sample?

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