The method uv irradiation-induced chemiluminescence (cl) is used in the tyrosine determination as this is as this enzyme can convert l-tyrosine to the nonchemiluminescent l-dopa.
Therefore, our novel pulse UV irradiation CL method for the determination of l-tyrosine was not only suitable for the determination of this vital amino acid but also extended to the successful determination of its producing and metabolizing enzymes and their inhibitors.
The proposed technique confirmed terrific sensitivity and ultraselectivity closer to l-tyrosine. The mechanism of the advanced CL technique become studied the usage of ROS scavengers, HPLC, and mass spectrometry. The technique become linear for l-tyrosine withinside the variety of 0.03-50 μM.
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