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Sagot :
The correct option is (a) oxygen consumption decreases.
Upon the addition of 2,4-dinitrophenol (DNP) to a suspension of mitochondria carrying out oxidative phosphorylation linked to the oxidation of malate, all of the following occur except: oxygen consumption decreases. oxygen consumption increases. the P/O ratio drops from a value of approximately 2.5 to 0.
The addition of 2, 4-dinitrophenol causes the uncoupling of the oxidative phosphorylation process and further causes a surge in the consumption of oxygen. Hence, it is an incorrect option.
As a result of the uncoupling effect caused by DNP, the oxygen consumption is enhanced but the process of ATP synthesis is affected. This is indicated by the drop in the P/O (phosphate/oxygen) ratio from 2.5 to 0. Hence, it is an incorrect option.
DNP causes the proton pumps to leak the proton back inside the mitochondrial matrix. This disturbs the proton gradient maintained across the mitochondria. Hence, it is an incorrect option.
When uncoupler agents are introduced into the mitochondria, the consumption of oxygen in mitochondria increases because of the increase in the rate of electron transfer. Hence, it is an incorrect option.
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